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Title: Lipid peroxidation and antioxidant enzyme activity in fresh rooster semen with normal and low sperm motility
Authors: Ngassa. Julius Mussa
Ruthaiporn Ratchamak
Thanaporn Ratsiri
Rujira Chumchai
Thevin Vongpralub
Wuttigrai Boonkum
Yoswaris Semaming
Vibuntita Chankitisakul
Keywords: antioxidant enzymes
enzyme activity
lipid peroxidation
sperm quality
Issue Date: 2020
Publisher: Faculty of Veterinary Medicine, Chiang Mai University
Citation: Veterinary Integrative Sciences (Vet Integr Sci) 18, 3 (Sep-Dec 2020), 183-192
Abstract: Lipid peroxidation is a propagative process which causes impaired motility, reduced acrosomal reaction, DNA damage, and cell apoptosis in spermatozoa of both mammals and avian species, including chickens. The aim of this study was to assess lipid peroxidation and enzyme activity of roosters semen having different sperm motilities. Semen was collected from 25 roosters which divided into two groups of roosters with normal (> 80%) and low sperm motility (< 80%). Semen quality parameters such as motility, concentration and volume, superoxide dismutase (SOD) and catalase (CAT) activities, and MDA concentrations between groups were measured. Sperm motility in roosters with normal and low motility was highly significant (P ≤ 0.001). However, volumes and concentrations between the two semen groups were not significantly different (P > 0.05). We observed an increase in MDA concentration in the group with low sperm motility, accompanied with a decrease in sperm motility, and vice versa in the normal motility group (P ≤ 0.05). However, activities of CAT and SOD did not significantly differ between the groups (P > 0.05). We therefore conclude that an increase in the level of lipid peroxidation in semen results in a decreased sperm motility in low-motility chickens and in an increase in normal-motility chickens. Similarities of the antioxidants appeared in our results are probably caused by different mechanisms employed by the ROS over the action of the antioxidant enzymes to protect sperm during lipid peroxidation.
Description: “Veterinary Integrative Sciences” is the official peer-reviewed journal of the Faculty of Veterinary Medicine, Chiang Mai University, Thailand. The primary aim of the journal is to facilitate and oversee the publication of a wide-range of high quality academic articles with an overall integration of the various areas of animal and veterinary sciences
ISSN: 2629-9968
Appears in Collections:CMUL: Journal Articles

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