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Title: Identification of Single Nucleotide Polymorphism Markers Associated with Northern Corn Leaf Blight Resistance in Sweet Corn
Authors: Kedsadaporn Junta
Orapin Saritnum
Ruangchai Juwattanasamran
Prawit Puddhanon
Darush Struss
Jumrearn Puttarach
Seksan Songchanthuek
Keywords: Northern corn leaf blight
Sweet corn
SNP markers
Issue Date: 2020
Publisher: Chiang Mai University
Citation: Chiang Mai University (CMU) Journal of Natural Sciences 19,1 (Jan-Mar 2020), p.155-162
Abstract: Northern corn leaf blight disease (NCLB) is a foliar disease of corn (Zea mays L.) caused by Exserohilum turcicum (Pass.). The Ht1, Ht2 and HtN1 genes in corn were found to control NCLB resistant traits. There has been an ongoing effort to identify various types of molecular markers associated with these genes in order to use them in marker-assisted selections. The objective of this study was to identify Single Nucleotide Polymorphism (SNP) markers which are associated with the Ht1 resistant gene. Ninety-three SNP markers were found in Ht1 regions, which are located on chromosome 2. Five SNP primers (MZSNP-0055106, MZSNP-0065744, MZSNP-0070164, MZSNP0063922, MZSNP-0073150) showed polymorphism between susceptible and resistant lines. The Chi-square test of genotypic data of 184 F2 plants (NT58WS6#4 x ChallengerS6-1) amplified by five markers fit a 1:2:1 ratio with Chi-square values of 0.82, 1.08, 1.08, 0.64 and 0.64 respectively. These 5 SNP primers may be useful as molecular markers for NCLB resistance in sweet corn.
Description: Chiang Mai University (CMU) Journal of Natural Sciences is dedicated to the publication of original research in Sciences & Technology and the Health Sciences. Submissions are welcomed from CMU, as well as other Thai and foreign institutions. All submissions must be original research not previously published or simultaneously submitted for publication. Manuscripts are peer reviewed using the double -blinded review system by at least 2 reviewers before acceptance. The CMU Journal of Natural Sciences is published four times a year, in January, April, July and October.
ISSN: 2465-4337
Appears in Collections:CMUL: Journal Articles

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