Please use this identifier to cite or link to this item: http://cmuir.cmu.ac.th/jspui/handle/6653943832/68232
Title: Antileukemic cell proliferation of active compounds from kaffir lime (Citrus hystrix) leaves
Authors: Songyot Anuchapreeda
Fah Chueahongthong
Natsima Viriyaadhammaa
Pawaret Panyajai
Riki Anzawa
Singkome Tima
Chadarat Ampasavate
Aroonchai Saiai
Methee Rungrojsakul
Toyonobu Usuki
Siriporn Okonogi
Keywords: Biochemistry, Genetics and Molecular Biology
Chemistry
Pharmacology, Toxicology and Pharmaceutics
Issue Date: 2-Mar-2020
Abstract: © 2020 MDPI AG. All rights reserved. Kaffir lime (Citrus hystrix) is a plant member of family Rutaceae, and its leaves are commonly used in folk medicine. The present study explores antileukemic effects of the extracts and purified active compounds from the leaves. The antileukemic activity was investigated via inhibition of Wilms' tumor 1 (WT1), which is a protein that involves in leukemic cell proliferation. In addition, the compounds were investigated for their effects on WT1 gene expression using real time RT-PCR and Western blotting. Cell cycle arrest and total cell number were investigated using flow cytometry and trypan blue exclusion method, respectively. The results demonstrated that the hexane fractionated extract had the greatest inhibitory effect on WT1 gene expression of many leukemic cell lines and significantly decreased WT1 protein levels of K562 cells (representative of the leukemic cells), in a dose- and time-dependent manner. Subfraction No. 9 (F9) after partial purification of hexane fractioned extract showed the highest suppression on WT1 protein and suppressed cell cycle at G2/M. The organic compounds were isolated from F9 and identified as phytol and lupeol. The bioassays confirmed antiproliferative activities of natural products phytol and lupeol. The results demonstrated anticancer activity of the isolated phytol and lupeol to decrease leukemic cell proliferation.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85081686177&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/68232
ISSN: 14203049
Appears in Collections:CMUL: Journal Articles

Files in This Item:
There are no files associated with this item.


Items in CMUIR are protected by copyright, with all rights reserved, unless otherwise indicated.