Please use this identifier to cite or link to this item: http://cmuir.cmu.ac.th/jspui/handle/6653943832/67981
Title: In vitro antifungal and antivirulence activities of biologically synthesized ethanolic extract of propolis-loaded PLGA Nanoparticles against Candida albicans
Authors: Anupon Iadnut
Ketsaya Mamoon
Patcharin Thammasit
Sudjai Pawichai
Singkome Tima
Kanya Preechasuth
Thida Kaewkod
Yingmanee Tragoolpua
Khajornsak Tragoolpua
Keywords: Medicine
Issue Date: 1-Jan-2019
Abstract: © 2019 Anupon Iadnut et al. Propolis is a natural substance and consists of bioactive compounds, which gives it antioxidant and antimicrobial properties. However, the use of propolis is limited by the low solubility in aqueous solutions. Thus, nanoparticles may be likely to accomplish enhanced delivery of poorly water-soluble phytomedicine. The aim of the present study was to fabricate and evaluate the biological activity of ethanolic extract of propolis-loaded poly(lactic-co-glycolic acid) nanoparticles (EEP-NPs). The EEP-NPs were prepared using the oil-in-water (o/w) single-emulsion solvent evaporation technique. The physicochemical properties of EEP-NPs were characterized and tested on their cytotoxicity, antifungal activity, and impact on key virulence factors that contribute to pathogenesis of C. albicans. EEP-NPs were successfully synthesized and demonstrated higher antifungal activity than EEP in free form. Moreover, EEP-NPs exhibited less cytotoxicity on Vero cells and suppressed the virulence factors of C. albicans, including adhesion, hyphal germination, biofilm formation, and invasion. Importantly, EEP-NPs exhibited a statistical decrease in the expression of hyphal adhesion-related genes, ALS3 and HWP1, of C. albicans. The results of this study revealed that EEP-NPs mediates a potent anticandidal activity and key virulence factors by reducing the gene-encoding virulence-associated hyphal- adhesion proteins of C. albicans and, thereby, disrupting the morphologic presence and attenuating their virulence.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85076780846&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/67981
ISSN: 17414288
1741427X
Appears in Collections:CMUL: Journal Articles

Files in This Item:
There are no files associated with this item.


Items in CMUIR are protected by copyright, with all rights reserved, unless otherwise indicated.