Please use this identifier to cite or link to this item: http://cmuir.cmu.ac.th/jspui/handle/6653943832/67643
Title: Valproic acid affects neuronal fate and microglial function via enhancing autophagic flux in mice after traumatic brain injury
Authors: Zhilong Zheng
Yanqing Wu
Zhengmao Li
Luxia Ye
Qi Lu
Yajiao Zhou
Yuan Yuan
Ting Jiang
Ling Xie
Yanlong Liu
Daqing Chen
Junming Ye
Wutigri Nimlamool
Hongyu Zhang
Jian Xiao
Keywords: Biochemistry, Genetics and Molecular Biology
Neuroscience
Issue Date: 1-Jan-2019
Abstract: © 2019 International Society for Neurochemistry In recent years, many studies have focused on autophagy, an evolutionarily conserved mechanism that relies on lysosomes to achieve cellular metabolic requirements and organelle turnover, and revealed its important role in animal models of traumatic injury. Autophagy is a double-edged sword. Appropriate levels of autophagy can promote the removal of abnormal proteins or damaged organelles, while hyperactivated autophagy can induce autophagic apoptosis. However, recent studies suggest that autophagic flux seems to be blocked after traumatic brain injury (TBI), which contributes to the apoptosis of brain cells. In this study, valproic acid (VPA), which was clinically used for epilepsy treatment, was used to treat TBI. The Morris water maze test, hematoxylin & eosin staining and Nissl staining were first conducted to confirm that VPA treatment had a therapeutic effect on mice after TBI. Western blotting, enzyme-linked immunosorbent assay and immunofluorescence staining were then performed to reveal that VPA treatment reversed TBI-induced blockade of autophagic flux, which was accompanied by a reduced inflammatory response. In addition, the variations in activation and phenotypic polarization of microglia were observed after VPA treatment. Nevertheless, the use of the autophagy inhibitor 3-methyladenine partially abolished VPA-induced neuroprotection and the regulation of microglial function after TBI, resulting in the deterioration of the central nervous system microenvironment and neurological function. Collectively, VPA treatment reversed the TBI-induced blockade of autophagic flux in the mouse brain cortex, subsequently inhibiting brain cell apoptosis and affecting microglial function to achieve the promotion of functional recovery in mice after TBI. (Figure presented.).
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85074554972&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/67643
ISSN: 14714159
00223042
Appears in Collections:CMUL: Journal Articles

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