The effect of semen collection interval on quality in frozen bull semen production

Abstract

Semen collection management is effected on semen quality of bull semen production. The objective of this study was to com-pare time interval of semen collection on fresh and frozen-thawed semen quality. The semen was collected from six Holstein Friesian bulls and Charolais bulls by using artificial vagina. The whole semen was divided into 2 groups: group 1: 5-day col-lection interval; and group 2: 2-day collection interval. The semen quality of fresh and frozen-thawed semen was evaluated under microscopic examination, using computer assisted semen analysis. The results of fresh semen showed that the 5-day collection interval group had significantly higher semen volume and concentration than the 2-day collection interval group (P<0.01). Furthermore, the semen quality of frozen-thawed semen showed that the 5-day collection interval group had better functional membrane integrity than the 2-day collection interval group (P<0.01). In conclusion, a long time semen collection interval might be better with respect to semen volume, semen concentration, and functional integrity of sperm membrane than that of a short time semen collection interval.