The First Genetic Linkage Map of Hypsizigus marmoreus Based on SCAR Marker

Abstract

This study presents the first genetic linkage map of the H. marmoreus constructed with 100 F1 population individuals using the sequence characterized amplified region (SCAR) base on the random amplified polymorphic DNA (RAPD) and the sequence-related amplified polymorphism (SRAP) techniques. In this study, a total of 14 polymorphic markers including 11 RAPD and 3 SRAP were used for the construction of the genetic linkage map. Five SCAR markers were grouped in two linkage groups, leaving 9 markers unlinked. The total length of the two linkage groups was 86.2 cM. This is the first genetic linkage map in the family Hypsizygus, which can be a basic framework for further mapping quantitative trait loci (QTL) linked to important biological traits of the H. marmoreus. It is also an important theoretical basis for the map-based cloning of related genes and for the breeding, classification, and identification of H. marmoreus. This map provides the reference information for future molecular breeding of H. marmoreus.