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Title: Diversity of bacteria nodulating Medicago arborea in the northeast area of Morocco
Authors: Kamal Guerrouj
Mustapha Bouterfas
Hanaa Abdelmoumen
Mustapha Missbah El Idrissi
Keywords: Medicago arborea
16S rDNA
bacterial diversity
Issue Date: 2016
Publisher: Science Faculty of Chiang Mai University
Citation: Chiang Mai Journal of Science 43, 3 (Apr 2016), 441 - 452
Abstract: A Polyphasic characterization of 30 bacteria isolated from root nodules of Medicago arborea (Medic tree) plants growing in 3 arid soils of the arid northeast region of Morocco was performed. The phenotypic, symbiotic, and cultural characteristics analyzed allowed the description of a broad physiological diversity among the isolates. The phenogram obtained showed 3 main clusters at a similarity coefficient of 78%. The results obtained suggest that the phenotype of these rhizobia might have evolved for adaptation to the local conditions. The genetic characterization which consisted in a rep PCR using ERIC (Enterobacterial Repetitive Intergenic Consensus) primers showed a great diversity and 3 groups and 8 clusters were delimited at a similarity level of 80%. However, the 16S rDNA sequences showed a perfect identity of all the isolates with Sinorhizobium meliloti published sequences. The results showed a relationship between rep-PCR fingerprinting and phenotypic techniques, which are complementary in diversity investigation and allowed revelation of relationships between M. arborea close-related bacterial isolates originated from different geographic location in the northeast area of Morocco.
ISSN: 0125-2526
Appears in Collections:CMUL: Journal Articles

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