Please use this identifier to cite or link to this item: http://cmuir.cmu.ac.th/jspui/handle/6653943832/65325
Title: Characterization of IgE-binding proteins in the salivary glands of Simulium nigrogilvum (Diptera: Simuliidae)
Authors: Chayanit Hempolchom
Nitat Sookrung
Wichai Srisuka
Onrapak Reamtong
Yuwaporn Sakolvaree
Wanpen Chaicumpa
Watcharatip Dedkhad
Narissara Jariyapan
Hiroyuki Takaoka
Atiporn Saeung
Keywords: Agricultural and Biological Sciences
Immunology and Microbiology
Medicine
Veterinary
Issue Date: 1-Jan-2019
Abstract: © 2019, Springer-Verlag GmbH Germany, part of Springer Nature. Simulium dermatitis is an IgE-mediated skin reaction in animals and humans caused by the bites of black flies. Although Simulium nigrogilvum has been incriminated as the main human-biting black fly species in Thailand, information on its salivary allergens is lacking. Salivary gland extract of S. nigrogilvum females was subjected to sodium dodecylsulfate-polyacrylamide gel electrophoresis, and the separated components were applied onto nitrocellulose membranes for immunoblotting, which was performed by probing the protein blots with sera from 17 individuals who were allergic to the bites of S. nigrogilvum. IgE-reactive protein bands were characterized further by liquid chromatography-mass spectrometry (LC-MS/MS) analysis. Nine protein bands (79, 42, 32, 25, 24, 22, 15, 13, and 11 kDa) were recognized in the serum of the subjects. Four of the nine protein bands (32, 24, 15, and 11 kDa) showed IgE reactivity in all (100%) of the tested sera, and they were identified as salivary secreted antigen 5-related protein, salivary serine protease, erythema protein, and hypothetical secreted protein, respectively. Three other proteins, salivary serine protease (25 kDa), salivary D7 secreted protein (22 kDa), and hypothetical protein (13 kDa), reacted with > 50% of the sera. The relevance of the identified protein bands as allergens needs to be confirmed by using pure recombinant proteins, either in the in vivo skin prick test or in vitro detection of the specific IgE in the serum samples of allergic subjects. This will be useful for the rational design of component-resolved diagnosis and allergen immunotherapy for the allergy mediated by the bites of black flies.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85069496609&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/65325
ISSN: 14321955
09320113
Appears in Collections:CMUL: Journal Articles

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