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dc.contributor.authorTeerarat Prasertseeen_US
dc.contributor.authorPhongsakorn Chuammitrien_US
dc.contributor.authorManu Deeudomen_US
dc.contributor.authorNipa Chokesajjawateeen_US
dc.contributor.authorPannita Santiyanonten_US
dc.contributor.authorPakpoom Tadeeen_US
dc.contributor.authorAniroot Nuangmeken_US
dc.contributor.authorPhacharaporn Tadeeen_US
dc.contributor.authorSamuel Shepparden_US
dc.contributor.authorBen Pascoeen_US
dc.contributor.authorPrapas Patchaneeen_US
dc.description.abstract© 2019 Salmonella enterica subsp. enterica serotype Rissen is the predominant serotype found in Thai pork production and can be transmitted to humans through contamination of the food chain. This study was conducted to investigate the genetic relationships between serovar Rissen isolates from all levels of the pork production chain and evaluate the ability of the in silico antimicrobial resistance (AMR) genotypes to predict the phenotype of serovar Rissen. A total of 38 serovar Rissen isolates were tested against eight antibiotic agents by a disk diffusion method and the whole genomes of all isolates were sequenced to detect AMR genetic elements using the ResFinder database.A total of 86.84% of the isolates were resistant to tetracycline, followed by ampicillin (78.96%) and sulfonamide-trimethoprim (71.05%). Resistance to more than one antimicrobial agent was observed in 78.95% of the isolates, with the most common pattern showing resistance to ampicillin, chloramphenicol, streptomycin, sulfonamide-trimethoprim, and tetracycline. The results of genotypic AMR indicated that 89.47% of the isolates carried tet(A), 84.22% carried blaTEM-1B, 78.95% carried sul3, and 78.95% carried dfrA12. The genotypic prediction of phenotypic resistance resulted in a mean sensitivity of 97.45% and specificity of 75.48%. Analysis by core genome multilocus sequence typing (cgMLST) demonstrated that the Salmonella isolates from various sources and different locations shared many of the same core genome loci. This implies that serovar Rissen has infected every stage of the pork production process and that contamination can occur in every part of the production chain.en_US
dc.subjectAgricultural and Biological Sciencesen_US
dc.subjectImmunology and Microbiologyen_US
dc.titleCore genome sequence analysis to characterize Salmonella enterica serovar Rissen ST469 from a swine production chainen_US
article.title.sourcetitleInternational Journal of Food Microbiologyen_US
article.volume304en_US of Bathen_US Universityen_US National Center for Genetic Engineering and Biotechnologyen_US Mai Universityen_US
Appears in Collections:CMUL: Journal Articles

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