Please use this identifier to cite or link to this item:
Full metadata record
DC FieldValueLanguage
dc.contributor.authorWilaiwan Phakthongen_US
dc.contributor.authorGillian M. Greenwayen_US
dc.contributor.authorNicole Pammeen_US
dc.contributor.authorSaisunee Liawruangrathen_US
dc.description.abstractA magnetic particles-based chemiluminescence immunoassay was investigated for progesterone detection by using luminometer. In this work, progesterone was determined based on the competitive binding between progesterone in the sample and progesterone-horseradish peroxidase (HRP) conjugate for a constant amount of rabbit anti-progesterone. Initially, anti-rabbit IgG coated magnetic particles conjugated with primary progesterone antibody were bound to progesterone in the samples. Then, the amount of progesterone was quantified by reacting with the residual unoccupied antibody sites with HRP-progesterone, followed by HRP substrate (luminol, H2O2, and p-iodophenol (PIP)) and finally detection of the generated chemiluminescence by a luminometer. The intensity of the emitting light was proportional to the amount of enzyme present (HRP-progesterone) and was inversely related to the amount of unlabeled progesterone in the sample. The optimum conditions for determination of progesterone were obtained at 0.15 µg L-1 magnetic particles, 5.0x10-4 mol L-1 luminol, 5.0x10-3 mol L-1 H2O2, 1.0x10-3 mol L-1 PIP, and phosphate buffer saline buffer pH 9. The optimal dilutions of both anti-progesterone antibody and HRP-progesterone conjugate were 1:1000. The linear relationship between chemiluminescence intensity (RLU) and various concentrations of progesterone was over the concentration range of 0.5-50.0 µg L-1. This proposed method had been successfully applied to the evaluation of progesterone in human sera.en_US
dc.publisherScience Faculty of Chiang Mai Universityen_US
dc.titleMagnetic Particles-based Chemiluminescence Immunoassay for Progesterone Determinationen_US
article.title.sourcetitleChiang Mai Journal of Scienceen_US
article.volume44en_US Flow Analysis Group, Department of Chemistry and center of Excellent for Innovation in Chemistry (PERCH-CIC) Together with Materials Science Research Center, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.en_US of Chemistry, The University of Hull, Cottingham Road, Hull HU6 7RX, United Kingdom.en_US of Pharmaceutical Science, Faculty of Pharmacy, Chiang Mai University, Chiang Mai, Thailand 50200, Thailand.en_US and Technology Research Institute, Chiang Mai University, Chiang Mai, 50200, Thailand.en_US
Appears in Collections:CMUL: Journal Articles

Files in This Item:
There are no files associated with this item.

Items in CMUIR are protected by copyright, with all rights reserved, unless otherwise indicated.