Optimization of Culture Conditions for Maximal Production of 4-vinyl Guaiacol from Ferulic Acid by Volvariella volvacea

Abstract

Ferulic acid has been of growing interest with regard to producing flavor compounds. In this study, the optimization conditions as substrate concentration, time of incubation, pH of the medium, age of inoculums and temperature of the bioconversion of ferulic acid into 4-vinyl guaiacol by Volvariella volvacea were determined. The results showed that the maximum production of 4-vinyl guaiacol (637.8 mg/L) was achieved under the following conditions; initial ferulic acid concentrations of 750 mg/L, 144 hours of incubation, pH of medium 7.5, age of inoculums 72 hours and incubation temperature 30 °C.