Please use this identifier to cite or link to this item:
Full metadata record
DC FieldValueLanguage
dc.contributor.authorSabadee Worrathampitaken_US
dc.contributor.authorShinji Tokuyamaen_US
dc.contributor.authorVichien Kitpreechavanichen_US
dc.contributor.authorSukhumaporn Sukkhumen_US
dc.description.abstractThis research demonstrated a new method to utilize nutrient composition in non-rubber skim latex for Poly (L-lactic acid) (PLA)-degrading enzymes production due to the enzyme is a very important trend regarding recycling plastic wastes in the future. Moreover, we attempted to find an alternative supporter such as scrub pad for cell immobilization and used as material for the enzyme production under open continuous fermentation. The PLA-degrading enzyme production was determined using a non-rubber skim latex and basal medium containing 0.035% (w/v) PLA powder and 1% (w/v) scrub pad as a supporter for the bacterial cells in a shake flask and fermenter. The effect of the physical parameters and the nitrogen sources were studied. The following optimal conditions were used: gelatin as the nitrogen source, a temperature of 45°C, an initial pH of 7.0, an agitation speed of 100 rpm, without aeration support and 3 days of cultivation. This resulted in an activity of approximately 41 U/ml and 0.56 U/ml.h of productivity in the 2 L fermenter. The highest PLA-degrading activity, 45 U/ml, was achieved in the fermenter by a continuous process under open fermentation with a dilution rate of 0.013 h-1. These results mean that non-rubber skim latex has the potential for PLA-degrading enzyme production through an open fermentation method in strain T16-1.en_US
dc.publisherScience Faculty of Chiang Mai Universityen_US
dc.titleUtilization of Non-rubber Skim Latex for Poly(L-lactide)-degrading Enzyme Production by Actinomadura keratinilytica Strain T16-1en_US
article.title.sourcetitleChiang Mai Journal of Scienceen_US
article.volume43en_US of Biology, Faculty of Science, Srinakharinwirot University, Watthana, Bangkok, 10110, Thailand.en_US of Biological Chemistry, Faculty of Agriculture, Shizuoka University, Shizuoka, Japan.en_US of Microbiology, Faculty of Science, Kasetsart University, Jatujak, Bangkok, 10900, Thailand.en_US
Appears in Collections:CMUL: Journal Articles

Files in This Item:
There are no files associated with this item.

Items in CMUIR are protected by copyright, with all rights reserved, unless otherwise indicated.