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Title: Evaluating of quality of rice bran protein concentrate prepared by a combination of isoelectronic precipitation and electrolyzed water treatment
Authors: Masanori Watanabe
Chikako Yamada
Isamu Maeda
Charin Techapun
Ampin Kuntiya
Noppol Leksawasdi
Phisit Seesuriyachan
Thanongsak Chaiyaso
Shinji Takenaka
Tadahiko Shiono
Kozo Nakamura
Shujiro Endo
Keywords: Agricultural and Biological Sciences
Issue Date: 1-Jan-2019
Abstract: © 2018 Elsevier Ltd A combination of isoelectronic precipitation and electrolyzed water treatment (IP-EWT) process was use for simultaneous recovery of protein and phosphorus compounds from heat-stabilized defatted rice bran (HSDFRB). The highest protein content (65.1 w/w%) recovery ratio (over 50%) of protein concentrate were attained by two-stage protein extraction with IP-EWT. Analysis of amino-acid composition demonstrated excellent amino acid value (76.6%) in the protein fraction than those of commercial cereals such as rice (61%), wheat (39%), and corn (31%). In addition, according to SDS-PAGE and immunoblotting analysis, neither rice allergenic protein nor heavy metals were detected in either HSDFRB or protein fractions extracted by IP-EWT. These results suggest that as an eco-friendly process, this combined (IP-EWT) process is suitable for practical recovery of highly concentrated and safe protein from HSDFRB without using enzymes or chemicals such as organic solvents, buffering agents, and surfactants.
ISSN: 00236438
Appears in Collections:CMUL: Journal Articles

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