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dc.contributor.authorBaisakhi Sahaen_US
dc.contributor.authorBenjaporn Chaiwunen_US
dc.contributor.authorDenice D. Tsao-Weien_US
dc.contributor.authorSusan L. Groshenen_US
dc.contributor.authorWesley Y. Naritokuen_US
dc.contributor.authorRoscoe D. Atkinsonen_US
dc.contributor.authorClive R. Tayloren_US
dc.contributor.authorS. Ashraf Imamen_US
dc.date.accessioned2018-09-10T04:08:04Z-
dc.date.available2018-09-10T04:08:04Z-
dc.date.issued2007-07-01en_US
dc.identifier.issn02771691en_US
dc.identifier.other2-s2.0-34250867496en_US
dc.identifier.other10.1097/01.pgp.0000250146.44592.d2en_US
dc.identifier.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=34250867496&origin=inwarden_US
dc.identifier.urihttp://cmuir.cmu.ac.th/jspui/handle/6653943832/61289-
dc.description.abstractThe expression of the catalytic subunit of telomerase protein (human telomerase reverse transcriptase [hTERT]), which is associated with telomerase activity, was evaluated as a potential marker of the high-grade premalignant cervical intraepithelial neoplasia (CIN 2/3) lesions. For comparison, cases of normal cervical squamous mucosa, low-grade CIN1 lesion, and cervical squamous cell carcinoma were included. The hTERT expression was also compared with Ki-67 and topoisomerase II-α (TPII-α) to determine the proliferative activity of the hTERT-positive dysplastic cells by a quantitative immunohistochemical staining method and was classified as follows: negative, 5% or less; moderate, 6% to 50%; or high, greater than 50% of the positive cells. The hTERT-positive cells were detected in a patchy pattern in the lower parabasal layers and in much of the basal layer in normal squamous mucosa. A similar frequency of Ki-67- or TPII-α-positive cells was observed, with the exception of the basal layer cells that were mostly negative. It is worthy to note that the recognizable intact basal layer cells in cases of CIN lesions were also consistently positive for the expression of hTERT, but rarely for Ki-67 or TPII-α. The expression of hTERT was detected in a less patchy pattern at a high or moderate percentage of the dysplastic epithelial cells each in 28.5% of cases of CIN1 lesions. A similar frequency, high and moderate percentage combined, of the TPII-α-positive dysplastic cell was also observed. In contrast, a high percentage of the hTERT-positive dysplastic cells were detected as diffuse basal or full-length thickness in 87.5% or 95% of cases of CIN2 or CIN3, respectively. A similar frequency of Ki-67 or TPII-α expression was observed in the dysplastic cells of CIN3 lesions. The pattern of hTERT-positive malignant cells in squamous cell carcinoma and dysplastic cells in the high-grade CIN lesions, to a greater extent, and dysplastic cells in the low-grade CIN lesion, to a lesser extent, was distinct from that of the normal cervical squamous mucosa. The results suggest that the progressive increase in the hTERT expression, together with the proliferative activity of the dysplastic epithelial cells of the high-grade CIN lesions, represents an early genetic abnormality in cervical pathogenesis. ©2007International Society of Gynecological Pathologists.en_US
dc.subjectMedicineen_US
dc.titleTelomerase and markers of cellular proliferation are associated with the progression of cervical intraepithelial neoplasia lesionsen_US
dc.typeJournalen_US
article.title.sourcetitleInternational Journal of Gynecological Pathologyen_US
article.volume26en_US
article.stream.affiliationsHuntington Medical Research Institutesen_US
article.stream.affiliationsChiang Mai Universityen_US
article.stream.affiliationsKeck School of Medicine of USCen_US
article.stream.affiliationsClarient, Inc.en_US
Appears in Collections:CMUL: Journal Articles

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