Please use this identifier to cite or link to this item: http://cmuir.cmu.ac.th/jspui/handle/6653943832/61143
Title: Intracellular growth and drug susceptibility of Mycobacterium tuberculosis in macrophages
Authors: Sakarin Chanwong
Niwat Maneekarn
Luksana Makonkawkeyoon
Sanit Makonkawkeyoon
Keywords: Immunology and Microbiology
Medicine
Veterinary
Issue Date: 1-Mar-2007
Abstract: Intracellular growth of Mycobacterium tuberculosis in human and rabbit monocytes and in mouse and guinea pig macrophages was evaluated. Monocytes or macrophages were infected with M. tuberculosis H37Rv with the multiplicity of infection at 10 mycobacteria per monocyte. The average percentages of infected human and rabbit monocytes were 22% and 19%, while mouse and guinea pig macrophages were 46% and 58%, respectively. The average generation times of M. tuberculosis H37Rv inside human and rabbit monocytes and in mouse and guinea pig macrophages, after culturing the infected cells for 10 days, were 33.4, 50.3, 31.4, and 25.6 h, respectively. Using infected guinea pig macrophages for intracellular evaluation of drug susceptibility, the minimal inhibitory concentrations (MICs) of isoniazid to the intracellular and extracellular M. tuberculosis H37Rv were 0.1 and 0.4 μg/ml, while the MICs of rifampicin were 0.1 and 0.2 μg/ml, respectively. The minimal bactericidal concentrations (MBCs) of isoniazid to the intracellular and extracellular H37Rv were 0.2 and 0.4 μg/ml, while the MCSs of rifampicin were 0.1 and 0.2 μg/ml, respectively. © 2006 Elsevier Ltd. All rights reserved.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=33846814925&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/61143
ISSN: 14729792
Appears in Collections:CMUL: Journal Articles

Files in This Item:
There are no files associated with this item.


Items in CMUIR are protected by copyright, with all rights reserved, unless otherwise indicated.