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Title: Overexpression of citrus polygalacturonase-inhibiting protein in citrus black rot pathogen Alternaria citri
Authors: Hiroshi Katoh
Sarunya Nalumpang
Hiroyuki Yamamoto
Kazuya Akimitsu
Keywords: Agricultural and Biological Sciences
Issue Date: 3-May-2007
Abstract: The rough lemon (Citrus jambhiri) gene encoding polygalacturonase-inhibiting protein (RlemPGIPA) was overexpressed in the pathogenic fungus Alternaria citri. The overexpression mutant AcOPI6 retained the ability to utilize pectin as a sole carbon source, and the overexpression of polygalacturonase-inhibiting protein did not have any effect on the growth of AcOPI6 in potato dextrose and pectin medium. The pathogenicity of AcOPI6 to cause a black rot symptom in citrus fruits was also unchanged. Polygalacturonase-inhibiting protein was secreted together with endopolygalacturonase into culture filtrates of AcOPI6, and oligogalacturonides were digested from polygalacturonic acid by both proteins in the culture filtrates. The reaction mixture containing oligogalacturonides possessed activity for induction of defense-related gene, RlemLOX, in rough lemon leaves. © 2006 Elsevier GmbH. All rights reserved.
ISSN: 01761617
Appears in Collections:CMUL: Journal Articles

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