Please use this identifier to cite or link to this item: http://cmuir.cmu.ac.th/jspui/handle/6653943832/60758
Title: A metabonomic analysis of insect development: <sup>1</sup>H-NMR spectroscopic characterization of changes in the composition of the haemolymph of larvae and pupae of the tobacco hornworm, Manduca sexta
Authors: Chitchol Phalaraksh
Stuart E. Reynolds
Ian D. Wilson
Eva M. Lenz
Jeremy K. Nicholson
John C. Lindon
Authors: Chitchol Phalaraksh
Stuart E. Reynolds
Ian D. Wilson
Eva M. Lenz
Jeremy K. Nicholson
John C. Lindon
Keywords: Multidisciplinary
Issue Date: 1-Sep-2008
Abstract: The analysis of complex biological samples, principally biological fluids, to obtain global metabolite profiles using techniques such as high resolution NMR spectroscopy has been termed metabonomics. This work describes the application of metabonomics to characterize the biochemical changes associated with development during the various instars of the larval stages and in the pupal stage of the tobacco hornworm Manduca sexta. The levels of many small molecule metabolites change during development. Thus, alanine, glutamate, lysine, succinate, lactate, and betaine are present at relatively high levels during the feeding period of larval life, but decrease at the onset of wandering. Compounds that increase in concentration as the insects prepare to pupate include citrate, fatty acids, and succinate. The presence of variable levels of putrescine in haemolymph is interesting, given its possible role in juvenile hormone action. The potential of high resolution 1H-NMR spectroscopy for the metabonomic analysis of insect developmental chemistry is discussed.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=53249149565&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/60758
ISSN: 15131874
Appears in Collections:CMUL: Journal Articles

Files in This Item:
There are no files associated with this item.


Items in CMUIR are protected by copyright, with all rights reserved, unless otherwise indicated.