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dc.contributor.authorNujira Tatunen_US
dc.contributor.authorTippawan Singtripopen_US
dc.contributor.authorJatuporn Tungjitwitayakulen_US
dc.contributor.authorSho Sakuraien_US
dc.date.accessioned2018-09-10T03:37:45Z-
dc.date.available2018-09-10T03:37:45Z-
dc.date.issued2008-08-01en_US
dc.identifier.issn09651748en_US
dc.identifier.other2-s2.0-46849090553en_US
dc.identifier.other10.1016/j.ibmb.2008.05.003en_US
dc.identifier.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=46849090553&origin=inwarden_US
dc.identifier.urihttp://cmuir.cmu.ac.th/jspui/handle/6653943832/60061-
dc.description.abstractDiapausing larvae of Omphisa fuscidentalis contain soluble and membrane-bound trehalase in the midgut. Soluble trehalase activity accounts for three-fourths of the total trehalase activity in midgut homogenates. The exposure of diapausing larvae to juvenile hormone analog (JHA) induced pupation, accompanied by an increase in soluble trehalase activity at the beginning of the prepupal period. Injection of 20-hydroxyecdysone (20E) increased the level of soluble trehalase activity 5 days postinjection in a dose-dependent manner. In contrast, no increase in membrane-bound trehalase activity was observed under the same conditions. We cloned the cDNAs that encode the soluble and membrane-bound forms of trehalase in O. fuscidentalis trehalase-1 (OfTreh-1) and trehalase-2 (OfTreh-2), respectively. Treh-1 encodes a 581-aa protein while Treh-2 encodes a 648-aa protein with one putative transmembrane domain near the C-terminus. The mRNA expression level of Treh-1 was 27-fold higher than that of Treh-2 in diapausing larval midgut. Following the exposure of diapausing larvae to JHA, Treh-1 mRNA expression increased gradually until the prepupal period whereupon it increased dramatically; in contrast, the mRNA expression of Treh-2 remained at its initial level. Similarly, 20E upregulated Treh-1 expression but had no effect on Treh-2 expression. Taken together, these results suggest that an increase in the soluble trehalase activity at pupation is caused by upregulation of Treh-1 gene. Moreover, membrane-bound trehalase does not appear to be involved in the dynamic changes in the hemolymph trehalose concentration that occur during the larval-pupal transformation. © 2008 Elsevier Ltd. All rights reserved.en_US
dc.subjectAgricultural and Biological Sciencesen_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.titleRegulation of soluble and membrane-bound trehalase activity and expression of the enzyme in the larval midgut of the bamboo borer Omphisa fuscidentalisen_US
dc.typeJournalen_US
article.title.sourcetitleInsect Biochemistry and Molecular Biologyen_US
article.volume38en_US
article.stream.affiliationsChiang Mai Universityen_US
article.stream.affiliationsKanazawa Universityen_US
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