Please use this identifier to cite or link to this item:
Title: NAD-malic enzyme affects nitrogen fixing activity of Bradyrhizobium Japonicum USDA 110 bacteroids in soybean nodules
Authors: Tan Van Dao
Mika Nomura
Rie Hamaguchi
Kensuke Kato
Manabu Itakura
Kiwamu Minamisawa
Suphawat Sinsuwongwat
Hoa Thi Phuong Le
Takakazu Kaneko
Satoshi Tabata
Shigeyuki Tajima
Keywords: Agricultural and Biological Sciences
Issue Date: 1-Dec-2008
Abstract: The NAD+-dependent malic enzyme (DME) has been reported to play a key role supporting nitrogenase activity in bacteroids of Sinorhizobium meliloti. Genetic evidence for a similar role in Bradyrhizobium japonicum USDA110 was obtained by constructing a dme mutant. Soybean plants inoculated with a dme mutant did not show delayed nodulation, but formed small root nodules and exhibited significant nitrogen-deficiency symptoms. Nodule numbers and the acetylene reducting activity per nodule as a dry weight value 14 and 28 days after inoculation with the dme mutant were comparable to those of plants inoculated with wild-type B. japonicum. However, shoot dry weight and acetylene reducting activity per nodule decreased to ca. 30% of the values in plants with wild-type B. japonicum. The sucrose and organic acid (malate, succinate, acetate, a-ketoglutarate and lactate) contents of the nodules were investigated. Amounts of sucrose, malate and a-ketoglutarate increased on inoculation with the dme mutant, suggesting that the decreased DME and nitrogenase activities in the bacteroids resulted in a reduction in the consumption of these respiratory metabolites by the nodules. The data suggest that the DME activity of B. japonicum bacteroids plays a role in nodule metabolism and supports nitrogen fixation.
ISSN: 13426311
Appears in Collections:CMUL: Journal Articles

Files in This Item:
There are no files associated with this item.

Items in CMUIR are protected by copyright, with all rights reserved, unless otherwise indicated.