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Title: Interaction of dengue virus envelope protein with endoplasmic reticulum-resident chaperones facilitates dengue virus production
Authors: Thawornchai Limjindaporn
Wiyada Wongwiwat
Sansanee Noisakran
Chatchawan Srisawat
Janjuree Netsawang
Chunya Puttikhunt
Watchara Kasinrerk
Panisadee Avirutnan
Somchai Thiemmeca
Rungtawan Sriburi
Nopporn Sittisombut
Prida Malasit
Pa thai Yenchitsomanus
Keywords: Biochemistry, Genetics and Molecular Biology
Issue Date: 6-Feb-2009
Abstract: Dengue virus infection is an important mosquito-borne disease and a public health problem worldwide. A better understanding of interactions between human cellular host and dengue virus proteins will provide insight into dengue virus replication and cellular pathogenesis. The glycosylated envelope protein of dengue virus, DENV E, is processed in the endoplasmic reticulum of host cells and therefore reliant on host processing functions. The complement of host ER functions involved and nature of the interactions with DENV E has not been thoroughly investigated. By employing a yeast two-hybrid assay, we found that domain III of DENV E interacts with human immunoglobulin heavy chain binding protein (BiP). The relevance of this interaction was demonstrated by co-immunoprecipitation and co-localization of BiP and DENV E in dengue virus-infected cells. Using the same approach, association of DENV E with two other chaperones, calnexin and calreticulin was also observed. Knocking-down expression of BiP, calnexin, or calreticulin by siRNA significantly decreased the production of infectious dengue virions. These results indicate that the interaction of these three chaperones with DENV E plays an important role in virion production, likely facilitating proper folding and assembly of dengue proteins. © 2008 Elsevier Inc. All rights reserved.
ISSN: 10902104
Appears in Collections:CMUL: Journal Articles

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