Please use this identifier to cite or link to this item: http://cmuir.cmu.ac.th/jspui/handle/6653943832/59188
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dc.contributor.authorMaslin Osathanunkulen_US
dc.contributor.authorKhukrit Osathanunkulen_US
dc.contributor.authorSutthipan Wongwanakulen_US
dc.contributor.authorRossarin Osathanunkulen_US
dc.contributor.authorPanagiotis Madesisen_US
dc.date.accessioned2018-09-05T04:41:13Z-
dc.date.available2018-09-05T04:41:13Z-
dc.date.issued2018-12-01en_US
dc.identifier.issn20452322en_US
dc.identifier.other2-s2.0-85052301557en_US
dc.identifier.other10.1038/s41598-018-31164-4en_US
dc.identifier.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85052301557&origin=inwarden_US
dc.identifier.urihttp://cmuir.cmu.ac.th/jspui/handle/6653943832/59188-
dc.description.abstract© 2018, The Author(s). Bar-HRM is a hybrid method which combines DNA barcoding and High Resolution Melting analysis. It has proven to be a fast, cost-effective and reliable molecular approach for species identification and authentication. Here, three aspects of the use of Bar-HRM are focused on. First, Bar-HRM is used to discriminate between closely related Ophiocordyceps species. Second, identification of an unknown powder that is claimed to be Ophiocordyceps species using Bar-HRM. Third, authenticating the O. sinensis products sold on the market by the Bar-HRM. Results from HRM analyses with ITS primers shows that the two Ophiocordyceps species (Ophiocordyceps sinensis and Ophiocordyceps militaris) were easily differentiated. Also, an unknown sample was able to be identified in less time compared with using DNA barcoding alone. In addition, the substitution or adulteration of O. sinensis products sold on market was detected via Bar-HRM. The substitution or adulteration of inferior Ophiocordyceps species, particularly O. militaris in high price O. sinensis products has been a concern throughout Asia. Based on our results, the Bar-HRM was again proved to be a promising tool for species identification and authentication.en_US
dc.subjectMultidisciplinaryen_US
dc.titleMultiuse of Bar-HRM for Ophiocordyceps sinensis identification and authenticationen_US
dc.typeJournalen_US
article.title.sourcetitleScientific Reportsen_US
article.volume8en_US
article.stream.affiliationsChiang Mai Universityen_US
article.stream.affiliationsPayap Universityen_US
article.stream.affiliationsMcCormick Hospitalen_US
article.stream.affiliationsCenter For Research And Technology - Hellasen_US
Appears in Collections:CMUL: Journal Articles

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