Please use this identifier to cite or link to this item: http://cmuir.cmu.ac.th/jspui/handle/6653943832/58248
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dc.contributor.authorSasiwan Kerdpooen_US
dc.contributor.authorWitida Laopajonen_US
dc.contributor.authorWatchara Kasinrerken_US
dc.contributor.authorSupansa Pataen_US
dc.contributor.authorThanusak Tatuen_US
dc.date.accessioned2018-09-05T04:21:40Z-
dc.date.available2018-09-05T04:21:40Z-
dc.date.issued2018-05-04en_US
dc.identifier.issn15324230en_US
dc.identifier.issn15321819en_US
dc.identifier.other2-s2.0-85049616434en_US
dc.identifier.other10.1080/15321819.2018.1488726en_US
dc.identifier.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85049616434&origin=inwarden_US
dc.identifier.urihttp://cmuir.cmu.ac.th/jspui/handle/6653943832/58248-
dc.description.abstract© 2018, © 2018 Taylor & Francis. Hemoglobin F (HbF) in blood lysate can be accurately measured by various methods, including immunoassay. In this study, we have produced polyclonal antibody (pAb) against HbF and established a modified sandwich-type ELISA for HbF quantification in blood lysates. The modified sandwich ELISA utilized anti-γ-globin monoclonal antibody clones Thal N/B as the capture antibody (Ab) coated on solid-phase, fluorescein isothiocyanate (FITC)-labeled pAb as the detecting Ab, and HPR-labeled anti-FITC Ab as the signal-generating Ab. By using an optimized blood lysate dilution, the HbF could be measured with no interference from hemoglobin Bart’s (Hb Bart’s) and hemoglobin Portland (Hb Portland 1) presented in α-thalassemia carriers. HbF levels measured by the modified sandwich ELISA were comparable to those quantified by the standard cation-exchange high performance liquid chromatography. We suggested that this modified sandwich ELISA was able to accurately measure HbF levels even in α-thalassemia carriers containing Hb Bart’s and Hb Portland 1 and be an alternative method for HbF measurement.en_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.subjectHealth Professionsen_US
dc.subjectImmunology and Microbiologyen_US
dc.subjectMedicineen_US
dc.titleA modified sandwich ELISA for accurate measurement of HbF in α-thalassemia carriers containing Hb Bart’s and Hb Portland 1en_US
dc.typeJournalen_US
article.title.sourcetitleJournal of Immunoassay and Immunochemistryen_US
article.volume39en_US
article.stream.affiliationsChiang Mai Universityen_US
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