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dc.contributor.authorAliki Xanthopoulouen_US
dc.contributor.authorIoannis Ganopoulosen_US
dc.contributor.authorPaschalitsa Tryfinopoulouen_US
dc.contributor.authorEfstathios Z. Panagouen_US
dc.contributor.authorMaslin Osanthanunkulen_US
dc.contributor.authorPanagiotis Madesisen_US
dc.contributor.authorDimosthenis Kizisen_US
dc.description.abstract© 2018 Society of Chemical Industry. BACKGROUND: Aspergillus is a diverse genus of fungi with high economic and social impact. Various species that belong to section Nigri (black aspergilli) are common agents of grape spoilage and potent producers of ochratoxin A (OTA), a mycotoxin associated with various nephrotoxic and immunotoxic effects in humans. Black aspergilli are difficult to classify following only phenotypic criteria; thus chemotaxonomic and molecular methods are employed in parallel with phenotypic ones for species characterization. These approaches, though accurate and replicable, require more than one individual step and are to a certain extent laborious when a rapid identification of these species is required. RESULTS: The aim of this study was to develop a high-resolution melting polymerase chain reaction (HRM-PCR) assay as a rapid method for identification of Aspergillus spp. section Nigri isolates and their detection in grape samples. Melt curve analysis of amplicons originating from the internal transcribed spacer 2 (ITS2) ribosomal region generated species-specific HRM curve profiles, enabling the accurate differentiation of the analyzed genotypes. Furthermore, the assay was able to identify A. carbonarius, A. tubingensis, A. niger, A. ibericus and A. japonicus in grape samples artificially inoculated with conidia of these fungi. CONCLUSION: To our knowledge this is the first report on the development of an HRM-PCR assay for the identification of black Aspergillus species in grape samples.en_US
dc.subjectAgricultural and Biological Sciencesen_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.titleRapid and accurate identification of black aspergilli from grapes using high-resolution melting (HRM) analysisen_US
article.title.sourcetitleJournal of the Science of Food and Agricultureen_US of Applied Biosciencesen_US of Plant Breeding and Genetic Resources ELGO-DEMETERen_US Panepistimion Athinonen_US Mai Universityen_US Phytopathological Instituteen_US
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