Please use this identifier to cite or link to this item:
Full metadata record
DC FieldValueLanguage
dc.contributor.authorKavi Ratanabanangkoonen_US
dc.contributor.authorPavinee Simsiriwongen_US
dc.contributor.authorKritsada Pruksaphonen_US
dc.contributor.authorKae Yi Tanen_US
dc.contributor.authorSukanya Eursakunen_US
dc.contributor.authorChoo Hock Tanen_US
dc.contributor.authorBunkuea Chantrathonkulen_US
dc.contributor.authorWongsakorn Wongwadhunyooen_US
dc.contributor.authorSirida Youngchimen_US
dc.contributor.authorNget Hong Tanen_US
dc.description.abstract© 2017 The Author(s). Snake envenomation is an important medical problem. One of the hurdles in antivenom development is the in vivo assay of antivenom potency which is expensive, gives variable results and kills many animals. We report a novel in vitro assay involving the specific binding of the postsynaptic neurotoxins (PSNTs) of elapid snakes with purified Torpedo californica nicotinic acetylcholine receptor (nAChR). The potency of an antivenom is determined by its antibody ability to bind and neutralize the PSNT, thus preventing it from binding to nAChR. The PSNT of Naja kaouthia (NK3) was immobilized on microtiter wells and nAChR was added to bind with it. The in vitro IC50of N. kaouthia venom that inhibited 50% of nAChR binding to the immobilized NK3 was determined. Varying concentrations of antisera against N. kaouthia were separately pre-incubated with 5xIC50of N. kaouthia venom. The remaining free NK3 were incubated with nAChR before adding to the NK3 coated plates. The in vitro and in vivo median effective ratio, ER50s of 12 batches of antisera showed correlation (R2) of 0.9809 (p < 0.0001). This in vitro assay should be applicable to antisera against other elapid venoms and should reduce the use of live animals and accelerate development of life-saving antivenoms.en_US
dc.titleA novel in vitro potency assay of antisera against Thai Naja kaouthia based on nicotinic acetylcholine receptor bindingen_US
article.title.sourcetitleScientific Reportsen_US
article.volume7en_US Research Instituteen_US Graduate Instituteen_US Universityen_US Mai Universityen_US of Malayaen_US
Appears in Collections:CMUL: Journal Articles

Files in This Item:
There are no files associated with this item.

Items in CMUIR are protected by copyright, with all rights reserved, unless otherwise indicated.