Systematic extraction and characterization of β-glucan from the industrial brewing yeast (Saccharomyces cerevisiae) waste material and the immunomodulatory studies on tumor necrosis factor-alpha and interleukin-6 cytokine balance of the mouse macrophage cell lines

Abstract

© 2017, Faculty of Pharmaceutical Sciences, Chulalongkorn University. All rights reserved. Objectives: The aim of this investigation was to create value to the industrial waste production by systematic extraction and purification of β-glucan (BBG) from the industrial brewing yeast (Saccharomyces cerevisiae) waste material. In addition, the immunological activity of the obtained BBG was also evaluated. Materials and Methods: The BBG from the yeast waste material of the brewing production was isolated by cell autolysis fractioning, solvent extraction, and drying. The obtained BBG was characterized by High-performance liquid chromatography, nuclear magnetic resonance and comparison with information in literature. The characterized BBG was then examined for its immunomodulatory activity on RAW 264.7 cell. Results: The optimal extraction method of BBG from the brewing waste material showed high extraction yield (13.14% w/w). In addition, the immunomodulatory study on RAW 264.7 cell lines showed that the isolated BBG could suppress the tumor necrosis factor-alpha and interleukin-6 production in the cell lines at 6 and 24 h, compared to the lipopolysaccharide control.