Please use this identifier to cite or link to this item: http://cmuir.cmu.ac.th/jspui/handle/6653943832/56847
Title: Hypermethylation of 28S ribosomal RNA in β-thalassemia trait carriers
Authors: Wannapa Sornjai
Pathrapol Lithanatudom
Jenny Erales
Philippe Joly
Alain Francina
Sabine Hacot
Suthat Fucharoen
Saovaros Svasti
Jean Jacques Diaz
Hichem C. Mertani
Duncan R. Smith
Keywords: Biochemistry, Genetics and Molecular Biology
Issue Date: 1-Jan-2017
Abstract: © 2016 Elsevier B.V. Ribosome biogenesis is the process of synthesis of the cellular ribosomes which mediate protein translation. Integral with the ribosomes are four cytoplasmic ribosomal RNAs (rRNAs) which show extensive post-transcriptional modifications including 2'-O-methylation and pseudouridylation. Several hereditary hematologic diseases including Diamond-Blackfan anemia have been shown to be associated with defects in ribosome biogenesis. Thalassemia is the most important hematologic inherited genetic disease worldwide, and this study examined the post-transcriptional ribose methylation status of three specific active sites of the 28S rRNA molecule at positions 1858, 4197 and 4506 of β-thalassemia trait carriers and normal controls. Samples from whole blood and cultured erythroid cells were examined. Results showed that site 4506 was hypermethylated in β-thalassemia trait carriers in both cohorts. Expression of fibrillarin, the ribosomal RNA methyltransferase as well as snoRNAs were additionally quantified by RT-qPCR and evidence of dysregulation was seen. Hemoglobin E trait carriers also showed evidence of dysregulation. These results provide the first evidence that ribosome biogenesis is dysregulated in β-thalassemia trait carriers.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84994000052&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/56847
ISSN: 18790003
01418130
Appears in Collections:CMUL: Journal Articles

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