Please use this identifier to cite or link to this item:
|Title:||Amperometric ascorbic acid biosensors based on the oxygen and glassy carbon electrodes modified with ascorbate oxidase immobilized-silk fibroin/polyethylene glycol membrane|
|Keywords:||Biochemistry, Genetics and Molecular Biology|
Physics and Astronomy
|Abstract:||© 2017, Chiang Mai University. All rights reserved. Amperometric biosensors for the specific determination of ascorbic acid (AA) were developed by immobilizing ascorbate oxidase (ASOD) on a polyethylene glycol (PEG) modified silk fibroin (SF) membrane coupled to either the O2electrode or the glassy carbon electrode (GCE). Both the O2 electrode and the GCE modified with SF/PEG/ASOD displayed their best sensor response at the same optimum conditions (0.40 mg/L of ASOD in phosphate buffer, pH 5). By comparing the responses of both AA biosensors, the GCE based biosensor showed better linearity (r2= 0.999 in the range of 0.5-12.0 mM) with shorter response time (26 s) compared to the O2electrode based biosensor (r2= 0.997 in the range of 0.5-5.0 mM, response time 48 s). In addition, the GCE based biosensor exhibited better selectivity and had simpler preparation steps. However, both biosensors provided approximately the same sensitivity, but the O2electrode based biosensor had a longer storage time (0.703 mA.mM-1, 9 days) than the GCE based one (0.695 mA.mM-1, 5 days). For both biosensors, there is little interference from citric acid, sodium chloride or ethanol, but some interference (2-4% error) from glucose.|
|Appears in Collections:||CMUL: Journal Articles|
Files in This Item:
There are no files associated with this item.
Items in CMUIR are protected by copyright, with all rights reserved, unless otherwise indicated.