Please use this identifier to cite or link to this item: http://cmuir.cmu.ac.th/jspui/handle/6653943832/56186
Title: Intermittent left cervical vagal nerve stimulation damages the stellate ganglia and reduces the ventricular rate during sustained atrial fibrillation in ambulatory dogs
Authors: Kroekkiat Chinda
Wei Chung Tsai
Yi Hsin Chan
Andrew Y.T. Lin
Jheel Patel
Ye Zhao
Alex Y. Tan
Mark J. Shen
Hongbo Lin
Changyu Shen
Nipon Chattipakorn
Michael Rubart-Von Der Lohe
Lan S. Chen
Michael C. Fishbein
Shien Fong Lin
Zhenhui Chen
Peng Sheng Chen
Keywords: Medicine
Issue Date: 1-Mar-2016
Abstract: © 2016 Heart Rhythm Society. All rights reserved. Background The effects of intermittent open-loop vagal nerve stimulation (VNS) on the ventricular rate (VR) during atrial fibrillation (AF) remain unclear. Objective The purpose of this study was to test the hypothesis that VNS damages the stellate ganglion (SG) and improves VR control during persistent AF. Methods We performed left cervical VNS in ambulatory dogs while recording the left SG nerve activity (SGNA) and vagal nerve activity. Tyrosine hydroxylase (TH) staining and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining were used to assess neuronal cell death in the SG. Results We induced persistent AF by atrial pacing in 6 dogs, followed by intermittent VNS with short ON-time (14 seconds) and long OFF-time (66 seconds). The integrated SGNA and VR during AF were 4.84 mV·s (95% confidence interval [CI] 3.08-6.60 mV·s) and 142 beats/min (95% CI 116-168 beats/min), respectively. During AF, VNS reduced the integrated SGNA and VR, respectively, to 3.74 mV·s (95% CI 2.27-5.20 mV·s; P =.021) and 115 beats/min (95% CI 96-134 beats/min; P =.016) during 66-second OFF-time and to 4.07 mV·s (95% CI 2.42-5.72 mV·s; P =.037) and 114 beats/min (95% CI 83-146 beats/min; P =.039) during 3-minute OFF-time. VNS increased the frequencies of prolonged (>3 seconds) pauses during AF. TH staining showed large confluent areas of damage in the left SG, characterized by pyknotic nuclei, reduced TH staining, increased percentage of TH-negative ganglion cells, and positive TUNEL staining. Occasional TUNEL-positive ganglion cells were also observed in the right SG. Conclusion VNS damaged the SG, leading to reduced SGNA and better rate control during persistent AF.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84958890232&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/56186
ISSN: 15563871
15475271
Appears in Collections:CMUL: Journal Articles

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