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dc.contributor.authorJeerang Wongtrakulen_US
dc.contributor.authorAtchara Paemaneeen_US
dc.contributor.authorPhitchayapak Wintachaien_US
dc.contributor.authorChutima Theppariten_US
dc.contributor.authorSittiruk Roytrakulen_US
dc.contributor.authorThananya Thongtanen_US
dc.contributor.authorKanokwan Janphenen_US
dc.contributor.authorKhuanchai Supparatpinyoen_US
dc.contributor.authorDuncan R. Smithen_US
dc.date.accessioned2018-09-05T03:09:33Z-
dc.date.available2018-09-05T03:09:33Z-
dc.date.issued2016-06-01en_US
dc.identifier.issn19957645en_US
dc.identifier.other2-s2.0-84966747159en_US
dc.identifier.other10.1016/j.apjtm.2016.04.015en_US
dc.identifier.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84966747159&origin=inwarden_US
dc.identifier.urihttp://cmuir.cmu.ac.th/jspui/handle/6653943832/56141-
dc.description.abstract© 2016 Hainan Medical College Objective To generate insights into the mechanism of NVP induced hepatotoxicity. Methods Liver (HepG2) cells were cultured with various concentrations of NVP. This cell line was chosen because it has low expression of cytochrome P450, allowing evaluation of the effects of NVP rather than specific metabolites. Cytotoxicity was determined using a proliferation assay and cell numbers were monitored using trypan blue exclusion assay for long term culture experiments and apoptosis induction was determined by morphological and biochemical investigation. Results HepG2 cells treated with the highest concentration of NVP tested (819 μM) initially showed a rounded morphology and all cells had died by week three of exposure. Nuclear condensation and fragmentation, increased Annexin V/propidium iodide staining and caspase 9 activation all supported the induction of apoptosis in HepG2 cells in response to NVP treatment. Conclusions There is a clear induction of apoptosis in response to NVP which suggests that NVP has significant cytotoxicity, over and above any cytotoxicity of metabolites and may contribute directly to patient hepatotoxicity.en_US
dc.subjectMedicineen_US
dc.titleNevirapine induces apoptosis in liver (HepG2) cellsen_US
dc.typeJournalen_US
article.title.sourcetitleAsian Pacific Journal of Tropical Medicineen_US
article.volume9en_US
article.stream.affiliationsChiang Mai Universityen_US
article.stream.affiliationsMahidol Universityen_US
article.stream.affiliationsThailand National Center for Genetic Engineering and Biotechnologyen_US
article.stream.affiliationsChulalongkorn Universityen_US
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