Please use this identifier to cite or link to this item: http://cmuir.cmu.ac.th/jspui/handle/6653943832/559
Title: Exopolysaccharides from lactic acid bacteria: Structural analysis, molecular weight effect on immunomodulation
Authors: Surayot U.
Wang J.
Seesuriyachan P.
Kuntiya A.
Tabarsa M.
Lee Y.
Kim J.-K.
Park W.
You S.
Issue Date: 2014
Publisher: Elsevier
Abstract: Exopolysaccharides (EPS) obtained from the culture medium of Lactobacillus confusus TISTR 1498 were investigated to determine their molecular characteristics and the effect of molecular weight (Mw) on immunomodulatory activity. The EPS mainly consisted of carbohydrates (81.9±2.4%) with only one type of monosaccharide, D-glucose, which was mostly connected by α-(1→6) glycosidic linkages. The EPS itself was unable to stimulate RAW264.7 cells to produce pro-inflammatory mediator nitric oxide (NO) and cytokines. However, considerable stimulation of RAW264.7 cells was observed by the low Mw of EPSs having Mw values≤70×103g/mol. The partially hydrolyzed EPS stimulated RAW264.7 cells to induce considerable NO and various cytokine production such as TNF-α, IL-1β, IL-6 and IL-10 via up-regulation of their mRNA expression. In addition, the degradation Iκ-B and the phosphorylation of c-Jun NH2-terminal kinase (JNK) were facilitated by BW-30 and MW-40, suggesting that the partially hydrolyzed EPS stimulated RAW264.7 cells through the activation of NF-κB and JNK pathways. © 2014 Elsevier B.V.
URI: http://www.scopus.com/inward/record.url?eid=2-s2.0-84900990109&partnerID=40&md5=47af0a7653837cad2c73e245640d3f2f
http://cmuir.cmu.ac.th/handle/6653943832/559
ISSN: 18790003
Appears in Collections:AGRO: Journal Articles

Files in This Item:
There are no files associated with this item.


Items in CMUIR are protected by copyright, with all rights reserved, unless otherwise indicated.