Streptomyces ferrugineus sp. nov., isolated from mangrove soil in Thailand

Abstract

© 2014 Springer International Publishing Switzerland Bacterial strain HV38Twas isolated from mangrove soil, which was collected from Thailand. Chemotaxonomic and morphological characteristics were found to be typical of members of the genus Streptomyces. The strain was found to form a distinct phyletic line in the Streptomyces 16S rRNA gene tree and to be closely associated with the type strains of Streptomyces coeruleofuscus CGMCC 4.1667T(98.84 % sequence similarity), Streptomyces chromofuscus CGMCC 4.1451T(98.63 %) and Streptomyces albidoflavus CGMCC 4.1291T(98.56 %). The major menaquinones were identified as MK-9(H8) and MK-9(H10). Its major cellular fatty acids were found to be iso-C14:0, iso-C15:0, anteiso-C15:0, iso-C16:1ω8c, C16:0, anteiso-C16:1ω8c, iso-C16:0and anteiso-C16:0. The DNA–DNA hybridization values between strain HV38Twith S. coeruleofuscus CGMCC 4.1667T, S. chromofuscus CGMCC 4.1451Tand S. albidoflavus CGMCC 4.1291Twere 32.7 ± 0.9, 21.8 ± 0.3 and 19.9 ± 0.9 %, respectively, which clearly supported the conclusion that they belong to separate genomic species. Cumulatively, the data indicated that strain HV38Trepresents a novel species of the genus Streptomyces, for which the name Streptomyces ferrugineus sp. nov. is proposed. The type strain is HV38T(=CCTCC AA2014009T= DSM 42152T).