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dc.contributor.authorHuayu Wuen_US
dc.contributor.authorUma Gauren_US
dc.contributor.authorSupamit Mekchayen_US
dc.contributor.authorXianwen Pengen_US
dc.contributor.authorLianghua Lien_US
dc.contributor.authorHua Sunen_US
dc.contributor.authorZhongxu Songen_US
dc.contributor.authorBinke Dongen_US
dc.contributor.authorMingbo Lien_US
dc.contributor.authorKlaus Wimmersen_US
dc.contributor.authorSiriluck Ponsuksilien_US
dc.contributor.authorKui Lien_US
dc.contributor.authorShuqi Meien_US
dc.contributor.authorGuisheng Liuen_US
dc.description.abstract© 2015, Institute of Plant Genetics, Polish Academy of Sciences, Poznan. Although allele expression imbalance has been recognized in many species, and strongly linked to diseases, no whole transcriptome allele imbalance has been detected in pigs during pathogen infections. The pathogen Streptococcus suis 2 (SS2) causes serious zoonotic disease. Different pig breeds show differential susceptibility/resistance to pathogen infection, but the biological insight is little known. Here we analyzed allele-specific expression (ASE) using the spleen transcriptome of four pigs belonging to two phenotypically different breeds after SS2 infection. The comparative analysis of allele specific SNPs between control and infected animals revealed 882 and 1096 statistically significant differentially expressed allele SNPs (criteria: ratio≧2 or ≦0.5) in Landrace and Enshi black pig, respectively. Twenty nine allelically imbalanced SNPs were further verified by Sanger sequencing, and later six SNPs were quantified by pyrosequencing assay. The pyrosequencing results are in agreement with the RNA-seq results, except two SNPs. Looking at the role of ASE in predisposition to diseases, the discovery of causative variants by ASE analysis might help the pig industry in long term to design breeding programs for improving SS2 resistance.en_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.titleGenome-wide identification of allele-specific expression in response to Streptococcus suis 2 infection in two differentially susceptible pig breedsen_US
article.title.sourcetitleJournal of Applied Geneticsen_US
article.volume56en_US Academy of Agricultural Sciencesen_US Key Laboratory of Animal Embryo Engineering and Molecular Breedingen_US Mai Universityen_US Institute for the Biology of Farm Animalsen_US Academy of Agricultural Sciencesen_US
Appears in Collections:CMUL: Journal Articles

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