Evaluation and application of a paper-based device for the determination of reactive phosphate in soil solution

Abstract

The evaluation and validation of a new low-cost microfluidic paper-based analytical device (μPAD) for the determination of reactive phosphate in soil solution is described. This device allows up to 15 replicate measurements of reactive phosphate on one credit card-sized device and requires only a desktop or hand scanner for signal detection and quantification. The proposed method showed a linear response between 0.1 and 1.0 mg L-1and between 1.0 and 10.0 mg L-1P with a limit of detection of 0.05 mg L-1P. When applied to the analysis of soil solution, there was excellent agreement between results obtained using the μPAD and those obtained by a reference spectrophotometric method, as indicated by the following regression equation: [P]μPAD= (0.997 ± 0.005)[P]Reference Method- (0.020 ± 0.008) (R2= 0.997; n = 110). It was found that the ambient temperature storage stability of the μPAD could be extended to 15 d by incorporating a removable polymeric interleaving sheet between the adjacent paper layers of the device. The observed sensitivity of the μPADs to sunlight, which was manifested by photoreduction of the chromogenic molybdate reagent used in the assay, was overcome by preparing the μPADs with an ultraviolet-filtering laminating material. The proposed method is rapid, with a reaction time of only 10 min, is easy to perform, and is suitable for application in the field. ©American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America.