Please use this identifier to cite or link to this item: http://cmuir.cmu.ac.th/jspui/handle/6653943832/53051
Title: Identification and characterisation of Aedes aegypti aldehyde dehydrogenases involved in pyrethroid metabolism
Authors: Nongkran Lumjuan
Jureeporn Wicheer
Posri Leelapat
Wej Choochote
Pradya Somboon
Keywords: Agricultural and Biological Sciences
Biochemistry, Genetics and Molecular Biology
Medicine
Issue Date: 21-Jul-2014
Abstract: Background: Pyrethroid insecticides, especially permethrin and deltamethrin, have been used extensively worldwide for mosquito control. However, insecticide resistance can spread through a population very rapidly under strong selection pressure from insecticide use. The upregulation of aldehyde dehydrogenase (ALDH) has been reported upon pyrethroid treatment. In Aedes aegypti, the increase in ALDH activity against the hydrolytic product of pyrethroid has been observed in DDT/permethrin-resistant strains. The objective of this study was to identify the role of individual ALDHs involved in pyrethroid metabolism. Methodology/Principal Findings: Three ALDHs were identified; two of these, ALDH9948 and ALDH14080, were upregulated in terms of both mRNA and protein levels in a DDT/pyrethroid-resistant strain of Ae. aegypti. Recombinant ALDH9948 and ALDH14080 exhibited oxidase activities to catalyse the oxidation of a permethrin intermediate, phenoxybenzyl aldehyde (PBald), to phenoxybenzoic acid (PBacid). Conclusions/Significance: ALDHs have been identified in association with permethrin resistance in Ae. aegypti. Characterisation of recombinant ALDHs confirmed the role of this protein in pyrethroid metabolism. Understanding the biochemical and molecular mechanisms of pyrethroid resistance provides information for improving vector control strategies. © 2014 Lumjuan et al.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84904562081&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/53051
ISSN: 19326203
Appears in Collections:CMUL: Journal Articles

Files in This Item:
There are no files associated with this item.


Items in CMUIR are protected by copyright, with all rights reserved, unless otherwise indicated.