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dc.contributor.authorLaddawan Potprommaneeen_US
dc.contributor.authorHaou Tzong Maen_US
dc.contributor.authorChein Hung Chenen_US
dc.contributor.authorPaitoon Aobcheyen_US
dc.contributor.authorKongsak Boonyapranaien_US
dc.contributor.authorBusyamas Chewaskulyongen_US
dc.contributor.authorPadchanee Sangthongen_US
dc.contributor.authorLalida Shanken_US
dc.contributor.authorShui Tein Chenen_US
dc.date.accessioned2018-09-04T09:21:43Z-
dc.date.available2018-09-04T09:21:43Z-
dc.date.issued2013-12-12en_US
dc.identifier.issn0974276Xen_US
dc.identifier.other2-s2.0-84889657116en_US
dc.identifier.other10.4172/jpb.1000289en_US
dc.identifier.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84889657116&origin=inwarden_US
dc.identifier.urihttp://cmuir.cmu.ac.th/jspui/handle/6653943832/52169-
dc.description.abstractHuman urine can be obtained from most patients by noninvasive ways of collection. It is a rich source of biomarkers for diagnostic tests, therapeutic guidance and prognostic information for patients and clinicians. Recently, the protein expression pattern of urine samples from lung cancer patients and healthy controls were compared using two-dimensional gel electrophoresis (2-DE). GM2 activator protein (GM2AP) in lung cancer patients with 2.5-4.0 folds higher than that found in healthy controls was the protein of interest, and this protein was focused because it is related to tumor-associated gangliosides found in cancer progression. The aim of this study was to investigate the urinary biomarkers associated with lung cancer. First, we applied proteomic approach to confirm the increase level of GM2AP in urine samples from lung cancer patients and healthy controls. Then, the expression level of urinary GM2AP was quantified using Western blotting and Enzyme-Linked Immunosorbent Assay (ELISA). The results show that the expression level of GM2AP significantly increased in each subtype of lung cancer patients (P<0.05). In addition, nano liquid chromatography coupled online with tandem mass spectrometry (NanoLC-MS/ MS) provided evidence of an increase of GM2AP with the glycan structure identification from doublely charge at m/z 1202.1025 corresponding to the structure of (Hex)3(HexNAc)2(Fuc)1 linked with peptide (PIIVOGNVTLSVVG). Overall data suggested that the GM2AP may be associated with lung cancer and may be useful as a marker to monitor lung cancer prognosis. © 2013 Potprommanee L, et al.en_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.subjectComputer Scienceen_US
dc.titleHuman urinary GM2-activator protein as a potential biomarker for lung canceren_US
dc.typeJournalen_US
article.title.sourcetitleJournal of Proteomics and Bioinformaticsen_US
article.volume6en_US
article.stream.affiliationsAcademia Sinica Taiwanen_US
article.stream.affiliationsChiang Mai Universityen_US
article.stream.affiliationsGenomics Research Center, Academia Sinicaen_US
article.stream.affiliationsNakhonratchasima Collegeen_US
article.stream.affiliationsNational Taiwan Universityen_US
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