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Please use this identifier to cite or link to this item: http://cmuir.cmu.ac.th/jspui/handle/6653943832/51422
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dc.contributor.authorDounghatai Singkaen_US
dc.contributor.authorLadapa Kumdhitiahutsawakulen_US
dc.contributor.authorPrasert Rekkriangkraien_US
dc.contributor.authorWasu Pathom-Areeen_US
dc.date.accessioned2018-09-04T06:01:43Z-
dc.date.available2018-09-04T06:01:43Z-
dc.date.issued2012-01-01en_US
dc.identifier.issn01252526en_US
dc.identifier.other2-s2.0-84869027526en_US
dc.identifier.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84869027526&origin=inwarden_US
dc.identifier.urihttp://cmuir.cmu.ac.th/jspui/handle/6653943832/51422-
dc.description.abstract© 2012, Chiang Mai University. All rights reserved. A protocol for DNA extraction directly from activated sludge is described. Sludge samples were lysed by glass bead and CTAB. The extracted DNA was purified with phenol: chloroform: isoamyl alcohol mixture and precipitated in sodium acetate and isopropanol. The proposed protocol is simple, rapid and required small sample size, yielding about 40.5 ± 1.8 μg DNA g-1sludge. The quality of DNA is good enough for successful PCR amplifications of 16S rDNA, 16S rDNA V3 region and denaturing gradient gel electrophoresis (DGGE) analysis of archaeal population. This described method can also be applied to other environmental samples such as whey and soil.en_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.subjectChemistryen_US
dc.subjectMaterials Scienceen_US
dc.subjectMathematicsen_US
dc.subjectPhysics and Astronomyen_US
dc.titleA simple method for DNA extraction from activated sludgeen_US
dc.typeJournalen_US
article.title.sourcetitleChiang Mai Journal of Scienceen_US
article.volume39en_US
article.stream.affiliationsChiang Mai Universityen_US
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