Please use this identifier to cite or link to this item:
Title: Gnathostoma spinigerum: Molecular cloning, expression and characterization of the cyclophilin protein
Authors: Porntip Laummaunwai
Pewpan M. Intapan
Chaisiri Wongkham
Viraphong Lulitanond
Chatchai Tayapiwatana
Wanchai Maleewong
Keywords: Immunology and Microbiology
Issue Date: 1-Dec-2010
Abstract: In this study, a cDNA encoding cyclophilin (CyP) of Gnathostoma spinigerum was cloned into a prokaryotic expression vector and expressed in Escherichia coli. The predicted molecular mass of the putative protein was 18.6. kDa, and the deduced amino acid sequence had 86, 84.8, 81.3 and 77.2% identity with the CyP of Dirofilaria immitis, Brugia malayi, Onchocerca volvulus and Caenorhabditis elegans, respectively. A prediction of linear B-cell epitopes with high hydrophilicity and immunoblotting results indicated that the recombinant CyP has antigenicity to humans. The recombinant CyP protein reacted with human gnathostomiasis sera but not with other parasitosis or healthy control sera, suggesting that it might be useful for the serodiagnosis of human gnathostomiasis. © Elsevier Inc.
ISSN: 10902449
Appears in Collections:CMUL: Journal Articles

Files in This Item:
There are no files associated with this item.

Items in CMUIR are protected by copyright, with all rights reserved, unless otherwise indicated.