Please use this identifier to cite or link to this item: http://cmuir.cmu.ac.th/jspui/handle/6653943832/50680
Title: Sequential injection-cation exchange micro-column system for hemoglobin typing to differentiate HbE carriers
Authors: Supaporn Kradtap Hartwell
Worathip Sripaoraya
Somchai Lapanantnoppakhun
Torpong Sanguansermsri
Kate Grudpan
Keywords: Chemistry
Medicine
Issue Date: 1-Mar-2010
Abstract: A weak cation exchange micro-column was incorporated into a sequential injection (SI) system to perform automatic hemoglobin (Hb) typing as an alternative way to measure HbE. Separation of HbF, HbA and HbA2/HbE was performed using phosphate buffer solutions in the pH range of 6 - 7 to create pH gradient mobile phase. The resultant chromatogram showed relative amounts of HbE to other types of hemoglobins in more quantitative detail than the conventional techniques such as dichlorophenol indophenol precipitation and micro-column anion exchange. The system is more economical than a commercially available ion-exchange HPLC analyzer for hemoglobin testing, though analysis time per run is longer due to the aspiration operation of the syringe pump of the SI system. It has been demonstrated that the system can differentiate negative (normal) from positive (HbE carriers) subjects. 2010 © The Japan Society for Analytical Chemistry.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=77953633916&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/50680
ISSN: 13482246
09106340
Appears in Collections:CMUL: Journal Articles

Files in This Item:
There are no files associated with this item.


Items in CMUIR are protected by copyright, with all rights reserved, unless otherwise indicated.