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dc.contributor.authorNuttaporn Pimphaen_US
dc.contributor.authorSaowaluk Chaleawlert-Umponen_US
dc.contributor.authorNuttapol Chruewkamlowen_US
dc.contributor.authorWatchara Kasinrerken_US
dc.description.abstractNovel immunomagnetic particles have been prepared for separation of CD4+lymphocytes. The magnetic nanoparticles with a diameter of approximately 5-6 nm were first synthesized by co-precipitation from ferrous and ferric iron solutions and subsequently encapsulated with poly(glycidyl methacrylate) (PGMA) by precipitation polymerization. Monoclonal antibody specific to CD4 molecules expressed on CD4+lymphocytes was conjugated to the surface of magnetic PGMA particles through covalent bonding between epoxide functional groups on the particle surface and primary amine groups of the antibodies. The generated immunomagnetic particles have successfully separated CD4+lymphocytes from whole blood with over 95% purity. The results indicated that these particles can be employed for cell separation and provide a strong potential to be applied in various biomedical applications including diagnosis, and monitoring of human diseases. © 2010 Elsevier B.V. All rights reserved.en_US
dc.titlePreparation of anti-CD4 monoclonal antibody-conjugated magnetic poly(glycidyl methacrylate) particles and their application on CD4<sup>+</sup>lymphocyte separationen_US
article.volume84en_US National Science and Technology Development Agencyen_US Mai Universityen_US
Appears in Collections:CMUL: Journal Articles

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