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Title: Inhibitory mechanism of pure curcumin on Wilms' tumor 1 (WT1) gene expression through the PKCα signaling pathway in leukemic K562 cells
Authors: Suwanna Semsri
Sheryl R. Krig
Lakmal Kotelawala
Colleen A. Sweeney
Songyot Anuchapreeda
Keywords: Biochemistry, Genetics and Molecular Biology
Issue Date: 21-Jul-2011
Abstract: The aim of this study was to investigate the inhibitory mechanism of pure curcumin on WT1 expression in leukemic K562 cells. Pure curcumin suppressed WT1 expression, independent of effects on protein degradation or WT1 mRNA stability. Chromatin immunoprecipitation and reporter gene assays indicate that pure curcumin treatment attenuates WT1 auto-regulation. Interestingly, PKCα inhibition mimicks the repressive effects of pure curcumin in K562 cells. Conversely, myristoylated PKCα over-expression increased WT1 expression and reversed the inhibitory effect of pure curcumin. Our study indicates that pure curcumin attenuates WT1 auto-regulatory function through inhibition of PKCα signaling in K562 cells. © 2011 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
ISSN: 18733468
Appears in Collections:CMUL: Journal Articles

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