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dc.contributor.authorU. Sanmeeen_US
dc.contributor.authorW. Piromlertamornen_US
dc.contributor.authorT. Vutyavanichen_US
dc.date.accessioned2018-09-04T04:04:48Z-
dc.date.available2018-09-04T04:04:48Z-
dc.date.issued2011-01-01en_US
dc.identifier.issn0093691Xen_US
dc.identifier.other2-s2.0-79961173231en_US
dc.identifier.other10.1016/j.theriogenology.2010.12.031en_US
dc.identifier.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=79961173231&origin=inwarden_US
dc.identifier.urihttp://cmuir.cmu.ac.th/jspui/handle/6653943832/49639-
dc.description.abstractWe studied the co-culturing effect of intact and half-destroyed 4-cell mouse embryos on blastocyst formation rate and cell counts. A laser beam was used to produce a hole and destroy an adjacent blastomere in two opposite areas of the zona in the experimental group (n = 342), and to open two opposite zonal holes in the controls (n = 318). Control and half-destroyed embryos were cultured together in varying ratios of 10:0, 7:3, 5:5, 3:7, and 0:10 (group 1-5, respectively) for 48 h in 10 μl drops of cleavage medium. They were then separated and cultured in blastocyst medium for 24 h. The results showed that half-destroyed embryos had no effect on the blastulation rates of controls (97-100%, P = 0.28). Neither was there a difference in the number of ICM (27.3 ± 6.7, 29.4 ± 9.9, 27.7 ± 9.3, 26.5 ± 6.4, in group 1-4, respectively; P = 0.491), TE (47.7 ± 18.6, 52.3 ± 13.9, 48.4 ± 19.2, 57.3 ± 12.9, in group 1-4, respectively; P = 0.101), nor total cells (75.0 ± 19.5, 81.3 ± 17.1, 76.1 ± 19.6, 83.7 ± 16.2, in group 1-4, respectively; P = 0.188) in the resulting blastocysts. However, among half-destroyed embryos, cleavage arrest decreased (58.3%, 39.6%, 17.9%, and 8.3%, in group 5 to 2, respectively; P < 0.001) and blastocyst development increased (38.3%, 58.2%, 72.6%, and 88.9%, in group 5 to 2, respectively; P < 0.001) following co-culturing with intact controls. These embryos had a higher number of ICM cells (P = 0.035), but no significant changes in TE (P = 0.262) and total cell counts (P = 0.065). The findings indicate that the co-culturing of half-destroyed with intact embryos increased the blastulation rate of the first but had no effect on the latter. © 2011 Elsevier Inc.en_US
dc.subjectAgricultural and Biological Sciencesen_US
dc.subjectVeterinaryen_US
dc.titleEffect of co-culturing of half-destroyed and intact 4-cell mouse embryos in varying ratios on subsequent in vitro developmenten_US
dc.typeJournalen_US
article.title.sourcetitleTheriogenologyen_US
article.volume75en_US
article.stream.affiliationsChiang Mai Universityen_US
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