Please use this identifier to cite or link to this item: http://cmuir.cmu.ac.th/jspui/handle/6653943832/45984
Title: การพัฒนาข้าวสายพันธุ์แท้โดยเทคนิดดับเบิลแฮพลอยด์
Other Titles: Development of Pure Line Rice by Double Haploid Techniques
Authors: ณัฐา โพธาภรณ์
ประสาทพร สมิตะมาน
ธีรยุทธ ตู้จินดา
โสภณ บุญธรรม
Keywords: ข้าวสายพันธุ์แท้
เทคนิดดับเบิลแฮพลอยด์
Issue Date: Aug-2556
Publisher: เชียงใหม่ : บัณฑิตวิทยาลัย มหาวิทยาลัยเชียงใหม่
Abstract: Rice breeding has been employed for the best varieties that high yielding, good grain quality, diseases and insects resistant are noted. Generally conventional breeding has been used by most breeders to create such varieties. However, in order to accomplishthis this goal, the pure line is required and usually it takes not less than 7-8 generations of selfing to obtain. An alternative way to produce pure line is to employ either anther or ovary culture for producing haploid (n=x) and double haploid (2n=2x) in rice. This process can produce homozygous line and rapid fixed recombination. It consumes less time and achievement. The ovaries and anthers of 10 backcross lines (BC3F6) of a cross Rathu Heenati/KDML 105 and 2 backcross lines (BC4F3) of a cross Rathu Heenati/KDML 105//Chai Nat 1 were cultured for callus induction. The results showed that calli from all lines of anther induced callus when cultured on N6 medium supplemented with 2 mg/L NAA, 2 mg/L 2,4-D, 3 mg/L kinetin, 500 mg/L casein hydrolysate and 50 g/L maltose, which gave the highest percentage of calli induction (16.23 and 15.67% from 325(3)-(1) and 237(4)-(1) lines, respectively). The highest percentage of calli induction (10.85%) from the ovary culture was obtained when cultured on N6 medium supplemented with 2 mg/L NAA, 2 mg/L 2,4-D, 1 mg/L kinetin, 500 mg/L casein hydrolysate and 40 g/L maltose. The calli from anther and ovary were regenerated to green plantlet on MS medium supplemented with 2 mg/L BAP, 0.5 mg/L NAA, 500 mg/L casein hydrolysate and 30 g/L sucrose. The percentage of calli development from ovary (7.46%) was greater than anther (3.75%) on same medium. Three and five plants derived from anther and ovary culture respectively were selected and proved to be haploid using chromosome counting, guard cell size, chloroplast number in the guard cell and plant height.
URI: http://cmuir.cmu.ac.th/jspui/handle/6653943832/45984
Appears in Collections:GRAD-Sciences and Technology: Theses

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ABSTRACT.pdfABSTRACT252.33 kBAdobe PDFView/Open    Request a copy
APPENDIX.pdfAPPENDIX364.38 kBAdobe PDFView/Open    Request a copy
CHAPTER 1.pdfCHAPTER 1240.66 kBAdobe PDFView/Open    Request a copy
CHAPTER 2.pdf CHAPTER 2387.4 kBAdobe PDFView/Open    Request a copy
CHAPTER 3.pdf CHAPTER 3500.98 kBAdobe PDFView/Open    Request a copy
CHAPTER 4.pdf CHAPTER 41.23 MBAdobe PDFView/Open    Request a copy
CHAPTER 5.pdfCHAPTER 5241.95 kBAdobe PDFView/Open    Request a copy
CHAPTER 6.pdfCHAPTER 6214.68 kBAdobe PDFView/Open    Request a copy
CONTENT.pdf CONTENT406.72 kBAdobe PDFView/Open    Request a copy
COVER.pdfCOVER609.84 kBAdobe PDFView/Open    Request a copy
REFERENCE.pdfREFERENCE258.73 kBAdobe PDFView/Open    Request a copy


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