Please use this identifier to cite or link to this item: http://cmuir.cmu.ac.th/jspui/handle/6653943832/38145
Title: Validation of the immunochromatographic strip for α-thalassemia screening: A multicenter study
Authors: Winichagoon P.
Kumpan P.
Holmes P.
Finlayson J.
Newbound C.
Kabral A.
Li B.
Nuinoon M.
Fawcett T.
Tayapiwatana C.
Kasinrerk W.
Fucharoen S.
Keywords: Medicine (all)
Biochemistry (medical)
Public Health, Environmental and Occupational Health
Issue Date: 1-Jan-2015
Publisher: Mosby Inc.
Abstract: © 2015 Elsevier Inc. All rights reserved. α<sup>0</sup>-Thalassemia occurs from a deletion of 2 linked α-globin genes and interaction of these defective genes leads to hemoglobin (Hb) Bart's hydrops fetalis, the most severe and lethal thalassemia syndrome. Identification of α<sup>0</sup>-thalassemia carriers is thus essential for the prevention and control program. An immunochromatographic (IC) strip test was developed for rapid screening of α<sup>0</sup>-thalassemia by testing for Hb Bart's in the blood samples using a specific monoclonal antibody against Hb Bart's. To evaluate its sensitivity and specificity, the IC strip test was assessed in a cohort with various thalassemia genotypes from 4 different laboratories in Thailand and Australia. The result showed 97% sensitivity in α-thalassemia carriers with 2 α-globin genes deletion and Hb H disease. This is, in particular, the useful rapid screening test for regions where β-thalassemia and homozygous Hb E are also common. Similar hematologic and Hb data make it impossible to address the concomitant inheritance of α<sup>0</sup>-thalassemia in these samples without polymerase chain reaction (PCR)-based techniques, leading to misdiagnosis of the risk of having Hb Bart's hydrops fetalis. However, α-globin genotyping should be carried out in samples with positive IC strip as positive reactivity was also observed in homozygous α<sup>+</sup>-thalassemia carriers who have 2 trans α-globin gene deletions. These results indicate that in combination with red blood cell indices, the IC strip test could rule out mass populations for further α<sup>0</sup>-thalassemia detection by PCR-based analysis. The Alpha Thal IC strip also has the potential to replace testing for Hb H inclusion bodies, as it appears to be more sensitive, specific, and less labor intensive.
URI: http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84929191929&origin=inward
http://cmuir.cmu.ac.th/handle/6653943832/38145
ISSN: 19315244
Appears in Collections:AMS: Journal Articles

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