Please use this identifier to cite or link to this item: http://cmuir.cmu.ac.th/jspui/handle/6653943832/1803
Title: Laboratory colonization of Aedes lineatopennis.
Authors: Jitpakdi A.
Junkum A.
Pitasawat B.
Komalamisra N.
Rattanachanpichai E.
Chaithong U.
Tippawangkosol P.
Sukontason K.
Puangmalee N.
Choochote W.
Issue Date: 2005
Abstract: Aedes lineatopennis, a species member of the subgenus Neomelaniconion, could be colonized for more than 10 successive generations from 30 egg batches [totally 2,075 (34-98) eggs] of wild-caught females. The oviposited eggs needed to be incubated in a moisture chamber for at least 7 days to complete embryonation and, following immersion in 0.25-2% hay-fermented water, 61-66% of them hatched after hatching stimulation. Larvae were easily reared in 0.25-1% hay-fermented water, with suspended powder of equal weight of wheat germ, dry yeast, and oatmeal provided as food. Larval development was complete after 4-6 days. The pupal stage lasted 3-4 days when nearly all pupae reached the adult stage (87-91%). The adults had to mate artificially, and 5-day-old males proved to be the best age for induced copulation. Three to five-day-old females, which were kept in a paper cup, were fed easily on blood from an anesthetized golden hamster that was placed on the top-screen. The average number of eggs per gravid female was 63.56 +/- 22.93 (22-110). Unfed females and males, which were kept in a paper cup and fed on 5% multivitamin syrup solution, lived up to 43.17 +/- 12.63 (9-69) and 15.90 +/- 7.24 (2-39) days, respectively, in insectarium conditions of 27 +/- 2 degrees C and 70-80% relative humidity.
URI: http://www.scopus.com/inward/record.url?eid=2-s2.0-33646166216&partnerID=40&md5=d4dbc86dfe91e9296c2868a11195b12f
http://cmuir.cmu.ac.th/handle/6653943832/1803
ISSN: 01251562
Appears in Collections:MED: Journal Articles

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