Please use this identifier to cite or link to this item: http://cmuir.cmu.ac.th/jspui/handle/6653943832/1752
Title: An optimized expression vector for improving the yield of dengue virus-like particles from transfected insect cells
Authors: Charoensri N.
Suphatrakul A.
Sriburi R.
Yasanga T.
Junjhon J.
Keelapang P.
Utaipat U.
Puttikhunt C.
Kasinrerk W.
Malasit P.
Sittisombut N.
Issue Date: 2014
Publisher: Elsevier
Abstract: Recombinant virus-like particles (rVLPs) of flaviviruses are non-infectious particles released from cells expressing the envelope glycoproteins prM and E. Dengue virus rVLPs are recognized as a potential vaccine candidate, but large scale production of these particles is hindered by low yields and the occurrence of cytopathic effects. In an approach to improve the yield of rVLPs from transfected insect cells, several components of a dengue serotype 2 virus prM. +. E expression cassette were modified and the effect of these modifications was assessed during transient expression. Enhancement of extracellular rVLP levels by simultaneous substitutions of the prM signal peptide and the stem-anchor region of E with homologous cellular and viral counterparts, respectively, was further augmented by codon optimization. Extensive formation of multinucleated cells following transfection with the codon-optimized expression cassette was abrogated by introducing an E fusion loop mutation. This mutation also helped restore the extracellular E levels affected negatively by alteration of a charged residue at the pr-M junction, which was intended to promote maturation of rVLPs during export. Optimized expression cassettes generated in this multiple add-on modification approach should be useful in the generation of stably expressing clones and production of dengue virus rVLPs for immunogenicity studies. © 2014 Elsevier B.V.
URI: http://www.scopus.com/inward/record.url?eid=2-s2.0-84902001370&partnerID=40&md5=1cd1e3045a0423ee62f7562b3ddf6966
http://cmuir.cmu.ac.th/handle/6653943832/1752
ISSN: 18790984
Appears in Collections:MED: Journal Articles

Files in This Item:
There are no files associated with this item.


Items in CMUIR are protected by copyright, with all rights reserved, unless otherwise indicated.